Study on the Role of Circular RNA hsa_circ_0001591 in Colorectal Cancer
DOI:
https://doi.org/10.18063/apm.v10i2.877Keywords:
Colorectal cancer, Whole transcriptome sequencing, Gene differential expression, Circular RNA, hsa_circ_0001591Abstract
Objective: To validate and characterize the expression of circular RNA hsa_circ_0001591—identified by high-throughput sequencing on the DNBSEQ platform—as differentially expressed in colorectal cancer (CRC) tissues versus adjacent non-tumor tissues and to explore its association with clinicopathological features and prognostic potential. Methods: RT-qPCR was performed on 13 paired CRC and matched adjacent samples to confirm elevated hsa_circ_0001591 levels, functional knockdown attempts were made in CRC cell lines with PCR verification of silencing efficiency, and statistical analyses were conducted to correlate RNA expression with CD31/CD34 status, patient age, and short-term postoperative outcomes. Results: Ten of 13 CRC specimens showed significantly higher hsa_circ_0001591 expression in tumor versus adjacent tissue, and elevated levels correlated with CD31/CD34 positivity (P = 0.041) and age > 70 years old (P = 0.033), whereas circRNA knockdown in vitro was inconclusive due to its inherent stability and no significant differences in short-term postoperative status were observed. Conclusion: hsa_circ_0001591 is upregulated in CRC and linked to markers of vascular invasion, suggesting a potential inhibitory role in angiogenesis, and its miRNA/mRNA interaction network may drive CRC pathogenesis, making it a promising molecular biomarker and therapeutic target.
References
Salmena L, Poliseno L, Tey Y, et al., 2011, A ceRNA Hypothesis: The Rosetta Stone of a Hidden RNA Language?. Cell, 146(3): 353–358.
Liu K, Yan Z, Li Y, et al., 2013, Linc2GO: A Human LincRNA Function Annotation Resource Based on ceRNA Hypothesis. Bioinformatics, 29(17): 2221–2222.
Li X, Yang L, Chen L, 2018, The Biogenesis, Functions, and Challenges of Circular RNAs. Mol Cell, 71(3): 428–442.
Xiao M, Ai Y, Wilusz J, 2020, Biogenesis and Functions of Circular RNAs Come into Focus. Trends Cell Biol, 30(3): 226–240.
Chen L, Yang L, 2015, Regulation of circRNA Biogenesis. RNA Biol, 12(4): 381–388.
Siegel R, Miller K, Goding A, et al., 2020, Colorectal Cancer Statistics, 2020. CA Cancer J Clin, 70(3): 145–164.
Wang Y, Wang Z, 2015, Efficient Backsplicing Produces Translatable Circular mRNAs. RNA, 21(2): 172–179.
Jeck WR, Sorrentino JA, Wang K, et al., 2013, Circular RNAs Are Abundant, Conserved, and Associated with ALU Repeats. RNA, 19(2): 141–157.
Schwanhäusser B, Busse D, Li N, et al., 2011, Global Quantification of Mammalian Gene Expression Control. Nature, 473(7347): 337–342.
Memczak S, Jens M, Elefsinioti A, et al., 2013, Circular RNAs Are a Large Class of Animal RNAs With Regulatory Potency. Nature, 495(7441): 333–338.
Scientific Platform Serving for Statistics Professional, 2021, SPSSPRO. (Version 1.0.11), Online Application Software, https://www.spsspro.com.
Hen L, 2020, The Expanding Regulatory Mechanisms and Cellular Functions of Circular RNAs. Nat Rev Mol Cell Biol, 21(8): 475–490.
