Progress in Applied Microbiology (PAM) is an international Open Access journal that publishes original research articles and review articles related to all areas of applied microbiology. The topics of interest include but not limited to applied microbial, cell physiology, genomics, proteomics, transcriptomics, taxonomy, classification, agricultural, industrial and food microbiology.
Exploration and Practical Research on Microbiology Blended Teaching Mode based on THEOL Platform
Microbiology stands as a pivotal foundational course within the vast domain of life sciences. Characterized by its deep theoretical underpinnings, microscopic abstractions, and inherent learning complexities, the subject often poses challenges to students. The exponential growth of information network technology and cloud computing, however, has ushered in new avenues for revolutionizing university course curricula. This advancement serves as a robust backbone for exploring and reforming blended teaching modalities that integrate online and offline learning experiences in microbiology. Our teaching plan design is anchored in the overarching educational principles of fostering morality, nurturing talents, and prioritizing student-centered development. Using Chapter 1, “Gram staining of bacteria,” as a case study, this paper delves into the teaching design, practical implementation, and assessment rubrics associated with employing the THEOL platform to facilitate a blended teaching approach in microbiology. By underscoring the imperative need for such reforms, we aim to bolster students’ capacity for continuous learning and foster a heightened sense of innovative development. Ultimately, this paves the way for cultivating a new generation of versatile, innovative talents.
Research Progress of Exosomal Proteomics in Plant-Microbe Interactions
Exosomes, containing various biological information molecules such as proteins, nucleic acids, lipids, and small molecule compounds, play a crucial role in plant-microbe interactions and are currently a cutting-edge focus in this research field. As carriers of multiple biological information molecules, the composition of exosomes determines their functions. Therefore, analyzing the protein composition of exosomes using proteomic techniques can provide insights into their role in plant-microbe interactions. This article systematically summarizes the research progress of exosomal proteomics in plant interactions with fungi and bacteria, emphasizing the correlation between exosomal protein composition and plant disease resistance signaling as well as pathogen virulence. Based on this, we propose future directions for exosomal proteomics research, aiming to provide a reference for a deeper understanding of the mechanisms underlying plant-microbe interactions.
Application of Proteomics and Metabolomics in Interstitial Lung Disease Research
Interstitial lung disease (ILD) is a heterogeneous group of disorders characterized by pathological changes such as inflammatory infiltration, fibrosis, and cell proliferation involving the lung interstitium. The clinical etiology of this disease is not yet fully understood, especially during acute exacerbations, where patients often have a poor prognosis. The combined use of proteomics and metabolomics holds promise as a powerful tool for screening novel biomarkers of ILD. This article reviews the application of proteomics and metabolomics techniques in the study of ILD, aiming to clarify the current research status and provide an outlook for future research directions.